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Conditions for the Utilization of Human Pluripotent Stem Cells Derived From Human Embryos.(Brief Article): An article from: Transplant News

This digital document is an article from Transplant News, published by Transplant Communications, Inc. on September 24, 2000. The length of the article is 2388 words. The page length shown above is based on a typical 300-word page. The article is delivered in HTML format and is available in your Amazon.com Digital Locker immediately after purchase. You can view it with any web browser.

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Atlas of Human Pluripotent Stem Cells: Derivation and Culturing (Stem Cell Biology and Regenerative Medicine)

Human pluripotent stem cells, including human embryonic stem cells and induced pluripotent stem cells, are a key focus of current biomedical research. The emergence of state of the art culturing techniques is promoting the realization of the full potential of pluripotent stem cells in basic and translational research and in cell-based therapies. This comprehensive and authoritative atlas summarizes more than a decade of experience accumulated by a leading research team in this field. Hands-on st

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National Institutes of Health Guidelines for Research Using Human Pluripotent Stem Cells NIH Guidelines.: An article from: Transplant News

This digital document is an article from Transplant News, published by Transplant Communications, Inc. on February 28, 2001. The length of the article is 516 words. The page length shown above is based on a typical 300-word page. The article is delivered in HTML format and is available in your Amazon.com Digital Locker immediately after purchase. You can view it with any web browser.

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Title: National Institutes of Health Guidelines for Research Using Human Pluripotent S

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Human Embryonic and Induced Pluripotent Stem Cells: Lineage-Specific Differentiation Protocols (Springer Protocols Handbooks)

Because of the huge potential of human embryonic stem (hES) cells, especially the newly developed human induced pluripotent stem (hiPS) cells, in disease treatment and life quality improvement, enormous efforts have been made to develop new methodologies to translate lab discoveries in stem cell research into bed-side clinical technologies. In Human Embryonic and Induced Pluripotent Stem Cells: Lineage-Specific Differentiation Protocols, experts in the field present a comprehensive collection of

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New Technique To Reprogram Cells With Risk Gene For Schizophrenia
Using skin cells from adult siblings with schizophrenia and a genetic mutation linked to major mental illnesses, Johns Hopkins researchers have created induced pluripotent stem cells (iPS cells) using a new and improved “clean” technique. Reporting online in Molecular Psychiatry, the team confirms the establishment of two new lines of iPS cells with mutations in the gene named Disrupted In …
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Johns Hopkins Team Creates Stem Cells From Schizophrenia Patients
Scientists use new technique to reprogram cells with risk gene for major mental illness Using skin cells from adult siblings with schizophrenia and a genetic mutation linked to major mental illnesses, Johns Hopkins researchers have created induced pluripotent stem cells (iPS cells) using a new and improved “clean” technique. Reporting online February 22 in Molecular Psychiatry, the team confirms …
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Scientists create stem cells from schizophrenia patients
Using skin cells from adult siblings with schizophrenia and a genetic mutation linked to major mental illnesses, researchers have created induced pluripotent stem cells using a new and improved “clean” technique.
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This is my video project for CBNS169 Winter 09 course. The purpose is to raise awareness of the politics/ethical issues of embryonic stem cell research as well as teach the audience a few facts about the current and ongoing research regarding pluripotent stem cells. Many references and parodies are used (most related to videogames) such as Super Robot Wars, Halo, Pokemon, Metal Gear Solid, the movie 300, and many more.
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Induced pluripotent stem cells differentiated in culture
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Bioethics and Stem Cell Research

Many people still think of bioethics as a subset of philosophy. Others hold that at least clinical bioethicsthe part devoted to decisions about patient careshould be thought of as a specialty within medicine and nursing. An example of how bioethical argument is constructed is provided by the recent debates over stem cell research. A brief discussion of this question also has the merit of indicating the role that religious arguments can play in bioethics. This is an important issue as there are some who would rather that religious views played no role in ethical thought, while there are others who believe that ethics is fundamentally underpinned by religion.
After a human egg and sperm join in fertilisation, an embryo begins to develop. By five days after conception, a hollow ball of cells has formed. The outer cell layer gives rise to the placenta. The inner 50 or so cells are known as pluripotent embryonic stem cells and go on to form the tissues of the developing embryo. Each of these embryonic stem cells can give rise to most cell types that make up an adult human body. As the embryo continues to develop, its cells become increasingly differentiated. Most of them lose the capacity to develop into a wide range of cells. Instead they become specialised, functioning as a red blood cell, one of the cell types in bone, or whatever. However, even in adults some cells naturally retain a certain capacity to give rise to a variety of different cell types. For example, blood stem cells, located in bone marrow, can develop into red blood cells, platelets and the various sorts of white blood cells.
One way in which stem cells might be used is by what is generally referred to as therapeutic cloning. In therapeutic cloning a patient needing a transplant would have one of their ordinary cells removed-this could simply be a cell from the base of a hair or any other suitable tissue. This cell, or its nucleus, would then be fused with an egg cell from which the nucleus had been removed.
This resulting cell would then be stimulated to divide in the same way that the cell that gave rise to Dolly the cloned sheep was. If all went to plan, stem cells could then be isolated from this developing embryo and encouraged to develop into tissues. It is hoped that these tissues could then be used for transplantation. For example, a person with Parkinson’s disease might receive a transplant of neural cells.
The principal ethical argument in favour of therapeutic human cloning is easily stated. It is that this technology stands a good chance of reducing human suffering and enhancing happiness. Indeed, it has been estimated that over one-third of the people in the United States are affected by diseases that may be helped by stem cell research (Perry, 2000, p. 1423). It may be that such benefits will be found to result from other technologies, for example through using stem cells derived from adults or from umbilical cords. However, most scientists believe that adult stem cells are likely to be less valuable for research and in developing new treatments than are the pluripotent stem cells that can only be derived from human embryos (Perry, 2000, p. 1423).
The main ethical arguments against therapeutic human cloning centre on questions to do with the status of the human embryo. Different people see the status of the human embryo very differently. Religious traditions hold varying views about the embryo’s value and status (Wertz, 2002, p. 208). Most of the ethical questions concerning the status of the embryo have long been examined in the context of abortion (Reiss, 2002, p. 70). Given that a widespread consensus on the moral status of the human embryo not only does not exist-and has probably not existed over many centuries of debate-but seems extremely unlikely to exist in the near future, the role of ethicists may be not so much to attempt to produce a definitive answer to the question of the status of the human embryo in the very early developmental stages at which therapeutic human cloning would take place, but more to help clarify arguments and indicate the implications of particular approaches.
For many centuries bioethics has concerned itself with such questions as ‘What is right and what is wrong?’, ‘Should we always tell the truth?’, ‘When, if ever, is it right to kill someone?’ Over the last few decades moral philosophers and others have realised how such questions generally assume that only human beings are objects of moral concern. With the rise in the environmental movement, the growing concern for the well-being of animals and the increasing importance of new biotechnologies, whether in medicine or agriculture, the term ‘bioethics’ is increasingly being used.

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In 2006 scientists learned how to create a pluripotent stem cell out of a human skin cell. These cells, called induced pluripotent stem (iPS) cells have many of the characteristics of embryonic stem cells including the ability to create mature cell types. Dr. Jerome Zack has a CIRM grant to create iPS cells that could be used in human therapies. Zack is Associate Director, UCLA AIDS Institute, professor of Medicine, Microbiology, Immunology & Molecular Genetics, and a scientist with the Eli and Edythe Broad Center of Regenerative Medicine and Stem cell Research at UCLA.
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